Skip to main content
. 2019 Jun 4;27(10):3019–3033.e5. doi: 10.1016/j.celrep.2019.05.025
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse anti-Armadillo (Arm) Developmental Studies Hybridoma Bank DSHB #N27A1; RRID:AB_528089
Mouse anti-Prospero (Pros) Developmental Studies Hybridoma Bank DSHB #MR1A; RRID:AB_528440
Rabbit anti-cleaved Death caspase 1 (Dcp1) Cell Signaling Cat. #9578; RRID:AB_2721060
Rabbit anti-phospho-Histone H3 (pH3) Cell Signaling Cat. #9701; RRID:AB_331535
Rabbit anti-phospho-p44/42 MAPK (dpERK) Cell Signaling Cat. #4370; RRID:AB_2315112
Goat anti-GFP pAb Abcam Cat. #ab6673; RRID:AB_305643
Cy3 AP Donkey Anti-mouse IgG Jackson ImmunoResearch Cat. #715-165-151; RRID:AB_2315777
Cy3 AP Donkey Anti-rabbit IgG Jackson ImmunoResearch Cat. #711-165-152; RRID:AB_2307443
Cy5 AP Donkey Anti-mouse IgG Jackson ImmunoResearch Cat. #715-175-151; RRID:AB_2340820
Cy5 AP Donkey Anti-rabbit IgG Jackson ImmunoResearch Cat. #711-175-152; RRID:AB_2340607
Cy2 AP Donkey Anti-goat IgG (H+L) Jackson ImmunoResearch Cat. #705-225-147; RRID:AB_2307341
Chemicals, Peptides, and Recombinant Proteins
4’,6-Diamidino-2-phenylindol Dihydrochlorid (DAPI) Carl Roth GmbH Cat. #6335
TRI Reagent Sigma-Aldrich Cat. #T9424
DNase I Thermo Scientific Cat. #AM2238
SuperScript III Reverse Transcriptase Thermo Scientific Cat. #18080044
GoTaq qPCR Master Mix Promega Cat. #A6000
DpnI New England Biolabs Cat. #R0176
T4 DNA Ligase (5 U/μl) Roche Cat. #799009
DpnII New England Biolabs Cat. #R0543
MyTaq HS DNA Polymerase Bioline Cat. #BIO-21112
AlwI New England Biolabs Cat. #R0513
Phusion High-Fidelity DNA Polymerase New England Biolabs Cat. #M0530
Paraquat Sigma-Aldrich Cat. #856177
1,4-Diazabicyclo[2.2.2]octane (DABCO) Sigma-Aldrich Cat. #D2522
Mowiol 4-88 Sigma-Aldrich Cat. #81381
Critical Commercial Assays
In Situ Cell Death Detection Kit, TMR red Roche Cat. #12156792910
Deposited Data
Ets21c- and PolII-specific TaDa-seq from esgTS and Myo1ATS adult midgut samples (Raw and processed data) This paper GEO: GSE122170
Experimental Models: Organisms/Strains
w1118 Bloomington Drosophila Stock Center BDSC ID: 3605; RRID: BDSC_3605
w; UAS-ets21cRNAi Vienna Drosophila Resource Center VDRC ID: 106153
w; UAS-ets21cRNAi #2 This paper N/A
w; UAS-ets21cWT M. Uhlirova (Külshammer et al., 2015) N/A
w; UAS-hepWT D. Bohmann BDSC ID: 9308; RRID: BDSC_9308
w; UAS-rasV12 T. Xu (Pagliarini and Xu, 2003) N/A
w; UAS-egfrACT F. Hamaratoglu (Queenan et al., 1997) N/A
w; UAS-p35 Bloomington Drosophila Stock Center BDSC ID: 5072; RRID: BDSC_5072
w; UAS-LT3-Dam A. H. Brand (Southall et al., 2013) N/A
w; UAS-LT3-Dam-Polymerase II A. H. Brand (Southall et al., 2013) N/A
w; UAS-LT3-Dam-ets21c-RA This paper N/A
w; UAS-impRNAi Vienna Drosophila Resource Center VDRC ID: 20322
w; UAS-upd3RNAi Vienna Drosophila Resource Center VDRC ID: 27136
w; UAS-pvf1RNAi Vienna Drosophila Resource Center VDRC ID: 102699
w; UAS-eip93FRNAi Bloomington Drosophila Stock Center BDSC ID: 57838; RRID: BDSC_57868
w; esg-GFPYB0232 L. O’Brien (Kelso et al., 2004) N/A
y w; esg-Gal4, UAS-GFP / CyO; tub-Gal80TS B. Biteau (Biteau et al., 2008) N/A
w; esg-Gal4, UAS-mCD8-GFP / CyO; UAS-H2B-RFP, tub-Gal80TS/ TM6B T. Reiff (Antonello et al., 2015) N/A
w; Myo1A-Gal4 / CyO; tub-Gal80TS, UAS-GFP / TM6B P. Patel (Jiang et al., 2009) N/A
y[1] M{vas-int.Dm}ZH-2A w∗;; P{CaryP}attP2 L. Partridge N/A
y w Act5C-Cas9 Bloomington Drosophila Stock Center BDSC ID: 54590; RRID: BDSC_54590
Oligonucleotides
Table S1 This paper Table S1
Recombinant DNA
pWIZ Drosophila Genomics Resource Center (Lee and Carthew, 2003) DGRC ID: 1008
pWIZ-ets21cRNAi #2 This study N/A
pUAST-attB-LT3-NDam A. H. Brand (Southall et al., 2013) N/A
pUAST-attB-LT3-Dam-ets21c-RA This paper N/A
Software and Algorithms
Fluoview 2.1c Software Olympus RRID:SCR_014215
Photoshop CS5.1 Adobe Systems, Inc. RRID:SCR_014199
cellSens Standard 1.11 Software Olympus RRID:SCR_016238
Prism 5 GraphPad Software RRID:SCR_002798
G∗Power Statistical Analysis Faul et al., 2009 RRID:SCR_013726
Fiji ImageJ RRID:SCR_002285
Excel Microsoft RRID:SCR_016137
TaDa analysis scripts T. Southall (Southall et al., 2013, Wolfram et al., 2012) N/A
Gene Ontology http://geneontology.org/ RRID:SCR_002811
Other
CFX96 Real-Time PCR System Bio-Rad N/A
FV1000 Confocal Microscope Olympus N/A
CKX41 Inverted Microscope Olympus N/A
Active Motif EpiShear Sonicator Active Motif Cat. #53052
HiSeq 2000 Illumina, Inc. N/A