Table 1.
Sample | Items | Result | |
---|---|---|---|
Cell culture supernatant (1st trial)a | Sterilization test | Bacteria | Negative |
Fungi | Candida albicans | ||
Mycoplasmal culture | Negative | ||
Mycoplasma test (PCR)b | Negative | ||
Endotoxin | 0.062 EU/mL | ||
Reagents for cultivation | Sterilization test | Bacteria | Negative |
Fungi | Negative | ||
Serum (patient) | Sterilization test | Bacteria | Negative |
Fungi | Negative | ||
Candida antigen | Negative | ||
Oral surface (patient) | Sterilization test | Fungi | Candida albicans |
Oral surface (operator 1) | Sterilization test | Fungi | Negative |
Oral surface (operator 2) | Sterilization test | Fungi | Negative |
Cell culture supernatant (2nd trial)a | Sterilization test | Bacteria | Negative |
Fungi | Negative | ||
Mycoplasmal culture | Negative | ||
Mycoplasma test (PCR)b | Negative | ||
Endotoxin | 0.136 EU/mL | ||
Oral surface (patient) | Sterilization test | Fungi | Candida albicans |
Cell culture supernatants were routinely used for quality control tests.
PCR for detecting Mycoplasma pneumoniae was performed in accordance with method shown by Jensen JS et al. [12].