Table 1.
The results of quality control tests.
| Sample | Items | Result | |
|---|---|---|---|
| Cell culture supernatant (1st trial)a | Sterilization test | Bacteria | Negative |
| Fungi | Candida albicans | ||
| Mycoplasmal culture | Negative | ||
| Mycoplasma test (PCR)b | Negative | ||
| Endotoxin | 0.062 EU/mL | ||
| Reagents for cultivation | Sterilization test | Bacteria | Negative |
| Fungi | Negative | ||
| Serum (patient) | Sterilization test | Bacteria | Negative |
| Fungi | Negative | ||
| Candida antigen | Negative | ||
| Oral surface (patient) | Sterilization test | Fungi | Candida albicans |
| Oral surface (operator 1) | Sterilization test | Fungi | Negative |
| Oral surface (operator 2) | Sterilization test | Fungi | Negative |
| Cell culture supernatant (2nd trial)a | Sterilization test | Bacteria | Negative |
| Fungi | Negative | ||
| Mycoplasmal culture | Negative | ||
| Mycoplasma test (PCR)b | Negative | ||
| Endotoxin | 0.136 EU/mL | ||
| Oral surface (patient) | Sterilization test | Fungi | Candida albicans |
a
Cell culture supernatants were routinely used for quality control tests.
b
PCR for detecting Mycoplasma pneumoniae was performed in accordance with method shown by Jensen JS et al. [12].