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. 2018 Jul 7;68(3):427–438. doi: 10.1007/s12031-018-1099-x

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — a Relative mRNA expression of target genes in interscapular brown adipose tissue (iBAT) of mice acclimated to thermoneutrality (30 °C) or cold (4 °C) for 3.5 weeks (n = 8/group). Expression was normalized to reference genes TBP and RPL19. Data are expressed as mean ± S.E.M. unless not detected (n.d.) and asterisks denote a significant temperature effect at α = 0.05 (30 °C vs. 4 °C). (b) Relative mRNA expression of PACAP receptors in adipocyte primary cultures from iBAT of non-acclimated mice (n = 3/group). Expression is normalized to the reference gene RPL19. Data are expressed as mean ± S.E.M., and asterisks denote a significant effect of differentiation at α = 0.05 (preadipocytes vs. differentiated, mature adipocytes)