Table 1.

Primer and probe sequences used in real-time quantitative PCR

Target Genes	Forward primer (5′-3′)	Reverse primer (5′-3′)	Annealing (°C)
UCP1	CCTGGCAGATATCATCAC	TCACCTTGGATCTGAAGG	52
HSL	GGAGCACTACAAACGCAACGA	TCGGCCACCGGTAAAGAG	55
ADRB3	CAACCCGGTCATCTACTG	ACCGTAGCTACACAGAAG	50
HOXC9	GCAGCAAGCACAAAGAGGA	CGTCTGGTACTTGGTGTAGGG	58
PACAP	ATCCAGCGGACAGGAGAGAT	TCCGAGTGGCGTTTGGTAAG	55
PAC1R	TTGATGACTATGAGCCCGAGT	ACAAGATGACCATGGCAGTG	57
VIP	CAGGAACCGGGAACAGACT	TATCAGGAATGCCAGGAACT	57
VPAC1	AAGTCATTGTAGAGGCAGAT	AATATGTCAAGACGGAATCAG	59
VPAC2	AGAGCCATCTCTGTGCTGGTCAA	AGGTAGGCCAGGAAGCAC	57
Hydrolysis probes (5′-3′)
VPAC2	56FAM/CAGGTAGAG/ZEN/ACCCTCCACCAGAAGCCAGTAG/3IABkFQ
PACAP	56FAM/TCGCCCACG/ZEN/AAATCCTTAACGAAGCCTATCGAA/3IABkFQ
Reference genes	Forward primer (5′-3′)	Reverse primer (5′-3′)
β-actin	GCTCTGGCTCCTAGCACCAT	GCCACCGATCCACACAGAGT	55
GAPDH	TGCACCACCAACTGCTTAG	GGATGCAGGGATGATGTTC	55
RPL19	CCATGAGTATGCTCAGGCTACAG	CTGATCTGCTGACGGGAGTTG	57.5
TBP	CACCAATGACTCCTATGA	CCAAGATTCACGGTAGATA	53
18S	CGGCTACCACATCCAAGGAA	GCTGGAATTACCGCGGCT	55