. 2019 Jun 18;2:215. doi: 10.1038/s42003-019-0474-7

Table 3.

Validation results of SNPs and indels of two species (P. alba and P.deltoides) by PCR and Sanger sequencing

Variation	Species	Variation predicted	Validation				Validation rate (%)^a	FP rate (%)^b	FN rate (%)^c
Variation	Species	Variation predicted	PCR	True	FP	FN	Validation rate (%)^a	FP rate (%)^b	FN rate (%)^c
SNPs	P. alba	186	177	173	13	4	93.01	6.99	2.15
	P.deltoides	84	87	84	0	3	100	0	3.57
	Total	270	264	257	13	7	95.19	4.81	2.59
Indels	P. alba	46	39	37	9	2	80.43	19.57	4.35
	P.deltoides	21	23	21	0	2	100	0	9.52
	Total	67	62	58	9	4	86.57	13.43	5.97

^aFP false positive, FP rate FP variations/variations detected by software × 100%

^bFN false negative, FN rate FP variations/variations detected by software × 100%

^cValidation rate True variations/variations detected by software × 10