HBsAg was downregulated in HCC tissues compared to tumor periphery. (A) Cases #1, 13, and 14 with negative signals from serological assays and viral DNA showed no HBsAg in liver tissues. (B, C) HBsAg positive HCC patients had different expression patterns in nontumor and tumor tissue. Distinctive features by cytoplasmic spots (#2 and 10), diffuse (#2, 3, 4, 5, 7, 9, 11, and 10) and membranous (#2, 5, 7, 9, and 12). Scale bars, 100 μm and 50 μm.