Fig. 3.

GST inhibition elicits a reduction in MCB fluorescence. Zebrafish embryos aged 48 hpf were exposed to 100 μM Ethacrynic Acid for 1 h (n = 21 fish) immediately prior to MCB staining and imaging. The fluorescence intensity was compared to 0.1% DMSO treated controls (n = 24 fish). Values are mean +SEM fluorescence intensities normalized to the mean fluorescence intensity of yolk of untreated controls, combined from at least 2 independent experimental runs. Stars indicate statistically significant differences (p ≤ 0.05), as determined by a t-test across the indicated structure. Right: Heatmaps of MCB fluorescence observed in embryos exposed to the stated treatment.