Fig. 4.

GSH modulation elicits a robust MCB response. Zebrafish embryos aged 48 hpf were exposed to 100 μM N-AcetylCysteine (NAC; n = 21 fish) or 5 μM Buthionine Sulfoximine (BSO; n = 28 fish), or a combination of the two (n = 40 fish) for 24 h prior to MCB staining and imaging. The fluorescence intensity was compared to untreated controls (n = 35 fish). Values are mean +SEM fluorescence intensities normalized to the mean fluorescence intensity of yolk of untreated controls, combined from at least 2 independent experimental runs. Different letters indicate statistically significant differences (p ≤ 0.05), as determined by a one-way ANOVA followed by a Tukey-Kramer post-hoc, across the indicated structure. Right: Heatmaps of monochlorobimane fluorescence observed in embryos exposed to the stated treatment.