Fig. 5.

MCB responds robustly to peroxide mediated GSH depletion. Zebrafish embryos aged 48 hpf were exposed to 750 μM tert-Butylhydroperoxide for 10 (n = 26 fish) or 30 (n = 25 fish) minutes immediately prior to MCB staining and imaging. The fluorescence intensity was compared to untreated controls (n = 27 fish). Values are mean +SEM fluorescence intensities normalized to the mean fluorescence intensity of yolk of untreated controls, combined from at least 2 independent experimental runs. Different letters indicate statistically significant differences (p ≤ 0.05), as determined by a one-way ANOVA followed by a Tukey-Kramer post-hoc, across the indicated structure. Right: Heatmaps of MCB fluorescence observed in embryos exposed to the stated treatment.