Fig. 8.

Developmental MEHP exposure induces structure and age dependent changes in MCB fluorescence. (A) Zebrafish embryos aged 48 hpf were exposed to 200 μg/L Mono(2-ethylhexyl) phthalate (MEHP) (n = 34 fish) or 0.01% DMSO (n = 23 fish) from 3 hpf until MCB staining and imaging. (B) Zebrafish embryos aged 72 hpf were exposed to 200 μg/L MEHP (n = 30 fish) or 0.01% DMSO (n = 24 fish) from 3 hpf until MCB staining and imaging. Values are mean +SEM fluorescence intensities normalized to the mean fluorescence intensity of yolk of untreated controls, combined from at least 2 independent experimental runs. Stars indicate statistically significant differences (p ≤ 0.05), as determined by a t-test, across the indicated structure. Right: Heatmaps of MCB fluorescence observed in embryos exposed to the stated treatment.