Fig. 5.

Transiently expressed XopR compromises the PCD induction on Nicotiana benthamiana. Agrobacterium tumefaciens strains bearing EYFP or EYFP::XopR fusion protein vector was inoculated into leaves (OD₆₀₀ = 1.0). Leaves were harvested at 3 dpi and photographed before (a) and after staining with trypan blue to indicate regions of PCD (b). Magnified microscopic view of the agro-infiltrated regions of leaves showing lesser dark blue patches, indicative of reduced cell death, in case of EYFP::XopR (c) as compared to empty vector control (d)