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. 2019 Jun 12;13:253. doi: 10.3389/fncel.2019.00253

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Copyright © 2019 Zhang, Li, Liu and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Toluidine blue staining and TEM of regenerated nerves 8 weeks after transplantation. Light microscopy images of toluidine blue staining of (A) the DMEM/F₁₂ group. (B) The EPI-NCSC group. (C) The OEC group, (D) the EPI-NCSC+OEC group, and (E) the Control group. (F) Analysis of the density of myelinated nerve fibers. TEM images of ultrathin sections showing myelinated nerve fibers in (G) the DMEM/F₁₂ group, (H) the EPI-NCSC group, (I) the OEC group, (J) the EPI-NCSC+OEC group, and (K) the Control group. (L) Analysis of the thickness of myelinated nerve fibers. A–D: Scale, 50 μm; E–H: Scale, 10 μm; Values are shown as mean ± SD (n = 5); N.S: no significant difference, ^∗P < 0.05; ^∗∗∗P < 0.001.