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. 2019 Jun 18;9:8682. doi: 10.1038/s41598-019-45095-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antiviral activity of niclosamide against DENV-1-4 in Huh-7 cells. (a) Representative dot plot analysis (FSC x 4G2-AF488) of Huh-7 cells infected with DENV-1, DENV-2, DENV-3, or DENV-4 in the presence of the indicated concentrations of niclosamide. The DENV-positive cell population is shown in the lower right quadrant. (b) Viral titres from cell culture supernatants were determined with a focus-forming assay. The data represent the means (±SD) of at least two independent experiments performed in duplicate. N.D., not detected. *p < 0.05, ***p < 0.001 and ****p < 0.0001 compared to DMSO control.