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. 2019 Jun 18;10:2685. doi: 10.1038/s41467-019-10555-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Mutations R719W and D382Y do not affect proximal S2 binding. Microscale thermophoresis of WT (black), R719W (blue), and D382Y (red) 2-hep HMM titrated with proximal S2. Representative data from one preparation of myosin, and three experimental repeats. Error bars represent the SEM. Source data are provided as a Source Data file