Fig. 3.

WT 25-hep HMM has lower ATPase activity than WT 2-hep HMM. a Mant-ATP single turnover kinetics of WT 2-hep HMM (black solid line and filled circles) and 25-hep HMM (red solid line and empty squares). Representative data from one preparation of each protein. Data is fit to a double exponential [Eq. (1)), with the slow phase in the 25-hep HMM clearly visible. Inset shows models of the 2-hep and 25-hep HMM, along with an SDS-PAGE gel showing the human heavy chain, the human ELC and the human RLC for both the 2-hep and 25-hep HMM (for uncropped image, see Supplementary Fig. 4). b Actin-activated ATPase activity of WT 2-hep HMM (black solid line and filled circles) and WT 25-hep HMM (red solid line and empty squares). Data is combined from two independent protein preparations with two experimental replicates for each preparation. Each point is an average with the error bar representing the SEM. Source data are provided as a Source Data file