Fig. 4.

Effect of mesa mutations R249Q and H251N on HMM ATPase kinetics. In all cases, black solid line and filled circles are 2-hep HMM data; red solid line and empty squares are 25-hep HMM data. a Mant-nucleotide single turnover kinetics for R249Q 2-hep HMM and 25-hep HMM. b Actin-activated ATPase data for R249Q 2-hep and 25-hep HMM. c Mant-nucleotide single turnover kinetics for H251N 2-hep and 25-hep HMM. d Actin-activated ATPase data for H251N 2-hep and 25-hep HMM. a, c show representative data from one preparation each. Each of panels b and d are combined data from three independent preparations of HMMs with three experimental replicates for each preparation. Each point is an average with the error bar representing the SEM. Source data are provided as a Source Data file