Fig. 5.

Effect of PHHIS-converter domain interaction mutations R719W and D382Y on HMM ATPase kinetics. In all cases, black solid line and filled circles are 2-hep HMM data; red solid line and empty squares are 25-hep HMM data. a Mant-nucleotide single turnover kinetics for R719W 2-hep and 25-hep HMM. b Actin-activated ATPase data for R719W 2-hep and 25-hep HMM. c Mant-nucleotide single turnover kinetics for D382Y 2-hep and 25-hep HMM. d Actin-activated ATPase data for D382Y 2-hep and 25-hep HMM. a, c show representative data from one preparation each. Each of panels b and d are combined data from two independent preparations of HMMs with three experimental replicates for each preparation. Each point is an average with the error bar representing the SEM. Source data are provided as a Source Data file