Fig. 7.

Effect of I457T mutation on HMM ATPase kinetics and in vitro motility velocity. The I457T mutation (green spheres) shown on the MS03 homology model²⁶ of the IHM. The mutation is located on the transducer region (pink ribbons) and is not close to the proposed interfaces within the IHM. b Close up of transducer region from panel a. c Mant-nucleotide release kinetics for I457T 2-hep and 25-hep HMM. d Actin-activated ATPase activity of I457T 2-hep and 25-hep HMM. e Bar plot showing the actin-activated ATPase activity of WT and I457T 2-hep HMM. f Bar plot showing the actin gliding velocity as measured by the in vitro motility assay of WT and I457T 2-hep HMM. In panels e andand f individual data points are denoted by closed circles. c Shows representative data from one preparation of each protein. Panel d is combined data from two independent protein preparations with three experimental replicates for each preparation. For panels e and f, each point is an average of two independent experiments (each from a different myosin preparation). Error bars represent the SEM. Source data are provided as a Source Data file