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. 2019 Jun 18;9:8688. doi: 10.1038/s41598-019-45131-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Percentage of accurate genotype calls for each DH sample based on the sequencing data from all 4 minION sequencing runs. Four different filtering treatments were applied: no filtering was performed (no dp filter), a minimum read depth of 2 and a maximum read depth of 5 (dp 2–5), removal of any heterozygous genotype calls in the minION sequences and without depth filtering (homozygous no dp filter) and removal of any heterozygous genotype calls in the minION sequences and a minimum read depth of 2 and a maximum read depth of 5 (homozygous dp 2–5).