Table 1.
Time (min) | Flow (μL/min) | Solvent A [0.1% formic acid in H2O (v/v)] | Solvent B [0.1% formic acid in acetonitrile (v/v)] | Comment |
---|---|---|---|---|
0–2 | 300 | 95% | 5% | Isocratic flow |
2–30 | 300 | 95–5% | 5–95% | Gradient flow |
30–40 | 300 | 5–95% | 95–5% | Isocratic flow |