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. 2019 Apr 22;47(11):5922–5935. doi: 10.1093/nar/gkz259

Table 3.

RT-PCR and qPCR primers

Gene Forward Reverse
LMNA (minigene) 5′-AAGAAGTGGCCATGCGCAAGC-3′ 5′-GGTGAACAGCTCCTCGCCCTT-3′
DsRed 5′-AGGACGGCTGCTTCATCTAC-3′ 5′-CCCATGGTCTTCTTCTGCAT-3′
LMNA (total endogenous) 5′-GTGTGGAAGGCACAGAACAC-3′ 5′-ACATGATGCTGCAGTTCTGG-3′
TBP 5′-AACAACAGCCTGCCACCTTA-3′ 5′-GCCATAAGGCATCATTGGAC-3′
qPCR Primers
LMNA (Δ150 isoform) 5′-GCGTCAGGAGCCCTGAGC-3′ 5′-GACGCAGGAAGCCTCCAC-3′
LMNA (WT isoform) 5′-CTGGTCACCCGCTCCTAC-3′ 5′-ACATGATGCTGCAGTTCTGG-3′
TBP 5′-AACAACAGCCTGCCACCTTA-3′ 5′-GCCATAAGGCATCATTGGAC-3′
DsRed 5′-AGGACGGCTGCTTCATCTAC-3′ 5′-CCCATGGTCTTCTTCTGCAT-3′