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. 2019 Apr 18;47(11):5936–5949. doi: 10.1093/nar/gkz270

Figure 1.

Figure 1.

Growth of the tRNA-intronless strains. (A) Growth of yeast strains in which all the tRNA genes encoding a certain tRNA species (shown on the right) were converted into an intronless allele was compared at various temperatures. Saturated cultures were serially diluted 10-fold as shown in the bottom, and 4 μl aliquots of each diluent were spotted onto YPD plates. The plates were incubated at the indicated temperatures. As described in the text, two wild-type strains were grown as controls: W303-1A as the parental strain of the tRNALeuUAG intronless strain and BY418 as the parental strain of the other intronless strains. Growth of TYSC2148 (ρ+ strain) is shown for the tRNALeuCAA intronless strain. (B) Growth of the tRNA intronless strains on different carbon sources. Serially diluted cultures of the two wild-type and 10 intronless strains were spotted onto YPGly (glycerol), YPEt (ethanol) and YPGal (galactose) plates as described in (A). The plates were incubated at 30°C.