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. 2019 Apr 18;47(11):5936–5949. doi: 10.1093/nar/gkz270

Figure 2.

Figure 2.

Growth curve of the tRNAPhe, tRNATyr, tRNAIleUAU and tRNALysUUU intronless mutants in liquid YPD or YPGly. (A) Saturated yeast cultures of the wild-type strain (BY418) and the four intronless strains of tRNAPhe, tRNATyr, tRNAIleUAU and tRNALysUUU were inoculated into the liquid YPD medium and grown at 30°C (left) or 23°C (right). The OD660 traces of the wild-type, tRNAPhe intronless and tRNATyr intronless strains were shown in the upper panels. Those of the wild-type, tRNAIleUAU intronless and tRNALysUUU intronless strains were in the lower panels. A color code and the doubling time under the log phase growth of each strain are indicated in the graph. (B) Similar experiments as those described in (A) were performed using the non-fermentable liquid medium, YPGly, instead of YPD. When the tRNAPhe intronless and tRNALysUUU intronless strains were cultured in YPGly, they showed biphasic growth defects so that their doubling time in each growth phase is indicated (a, Early phase; b, Late phase).