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. 2019 Apr 18;47(11):5936–5949. doi: 10.1093/nar/gkz270

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Intron removal from some isoacceptor tRNAs has weak effects on expression of the corresponding tRNAs. Northern blotting of tRNA levels in the wild-type (W) and intronless (Δ) strains. Small RNA fractions (2.0 μg each) were analyzed by TBE-based urea-PAGE/northern blotting for each tRNA isotype. The expression level of a major tRNA, tRNA^Asp, was used as an internal control. The upper bar graph shows quantification of the northern blotting data. The amount of a tRNA species shown in the graph bottom was first normalized by that of tRNA^Asp, and is expressed as relative abundance where the normalized amount of each tRNA in the wild-type strain is set to 1. Data represent the mean plus standard deviation (error bar) of at least three biological replicates. The lower images show a set of typical northern blotting images of the RNA in question (upper) and the control tRNA^Asp (lower); *, P < 0.05 and **, P < 0.001 by Student's t-tests.