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. 2019 Apr 24;47(11):5777–5791. doi: 10.1093/nar/gkz301

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Mitochondrial localization of selected candidates tested by a biochemical assay (A) List of tested candidates. Description as for Figure 2A. (B) Western blot analysis of total (T) yeast extracts and fractionated into cytosolic (C) and mitochondrial (M) or dense mitochondrial (D, 8 times concentrated) fractions. C-terminally TAP-tagged proteins were detected using peroxidase-anti-peroxidase (PAP) antibodies; Trr1, Ilv2 (mitochondrial control) and Sgt2 (cytoplasmic control) with specific antibodies. (C) AUG-initiated variants of Mtr4 and Lsm1 do not localize to mitochondria. Western blot analysis of cytosolic (C) and mitochondrial (M, D) fractions from yeast strains expressing N-terminally HA-tagged proteins from the major AUG codon using anti-HA (haemagglutinin) antibody. (D) Ribosome density A-site profiles along mRNAs coding for tested proteins. Description as for Figure 2D.