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. 2019 Apr 8;47(11):5698–5711. doi: 10.1093/nar/gkz242

Figure 2.

Figure 2.

Perturbations that rescue ΔdnaA lethality. Isogenic ΔdnaA derivatives (also bearing tus rpoB*35 mutations for all but that shown in panel xii) carrying dnaA+ shelter plasmid pHYD2388 (or pHYD4805, in case of recG mutant) and the additional genetic perturbation(s) as indicated on the top of each panel were plated on LB-Xgal plates and incubated for 40 hours at 37°; representative images are shown, and the numbers beneath each of the panels indicate the percentage of white colonies to the total, that is, viable even in absence of dnaA+ shelter plasmid (minimum of 500 colonies counted). Examples of white colonies and white-sectored blue colonies are marked by the white and black arrows, respectively. Strains employed for the different panels were pHYD2388 (or pHYD4805, in case of recG mutant) derivatives of: i, GJ16459; ii, GJ16475; iii, GJ16464; iv, GJ16445; v, GJ16488; vi, GJ18625; vii and viii, GJ16488 with pCA24N and pASKA-dam+, respectively; ix, GJ16469; x, GJ18626; xi, GJ18613; and xii, GJ18628. The medium was supplemented with Ara at 0.2% for panels x and xi.