Figure 3.

CbAgo associates with small plasmid derived siDNA in vivo. (A) Nucleic acids that co-purified with CbAgo were treated with either RNAse A, DNAse I or both, and were analyzed by denaturing polyacrylamide gel electrophoresis. (B) Histogram displaying the length of DNA co-purified with CbAgo as determined by sequencing. (C) Sequenced nucleic acids that co-purified with CbAgo are mostly complementary to the CbAgo expression plasmid. (D) Heat map showing the base preference of the co-purified nucleic acids at each position. The red squares indicate bases that were more often found compared to a random distribution (25%); blue squares indicate bases that were less frequently found.