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. 2019 Apr 2;47(11):5684–5697. doi: 10.1093/nar/gkz228

Figure 3.

Figure 3.

BUD32 deletion impairs DNA end resection. (A) Schematic representation of ssDNA measurement by Southern blot and real-time PCR. Green arrow pair shows the primers used for real-time PCR. Cyan bar shows the location of probe used for hybridization. (B) FACS analysis of cell cycle progression. HO expression was induced in cells cultured to log-phase stage. Cell samples were taken every one hour and FACS was performed to analyze the cell cycle progression. (C) Southern blot analysis of StyI digested genomic DNA isolated from indicated strains collected at different time points. (D) Plot showing the percentage of resected DNA by real-time PCR. Data are means ± s.e.m. (n = 3). (E) Rfa1–13Myc enrichment at the HO-induced break site. ChIP assay with anti-Myc antibody (+Ab) or without anti-Myc antibody (–Ab) was performed to measure the recruitment of Rfa1–13Myc protein at DNA sites located 0.5, 1, 3.7, 5 and 10 kb away from the break. Results are fold enrichment when HO was induced for 2.5 hr over that at time 0 hr (n = 2).