Figure 4.

KEOPS promotes homologous recombination. (A) Schematic representation of a plasmid-mediated HR assay. If the EcoRI linearized plasmid is introduced in yeast cells of BY4741/42, the cells can survive on histidine minus medium only when the plasmid is repaired through HR by using the genomic his3Δ1 DNA as a donor sequence. (B) Recombination rates in indicated strains. Recombination rates were assayed with the system described in A. The results are presented as the means ± s.e.m. (n ≥ 3). *** represents P < 0.001, * represents P < 0.05 (Student's t-test). (C) Schematic representation of ectopic recombination. (D) Viability of indicated strains after induction of a DSB as shown in C. n ≥ 4.