Figure 8.

Model depicting the function of KEOPS at DSB. When a DSB occurs, In addition to Yku70/80 and MRX, KEOPS complex also functions as a sensor, and is recruited to the break sites. The binding of Yku complex prevents the access of Exo1 and Dna2/Sgs1 to DNA breaks. The binding of MRX–Sae2 and KEOPS antagonizes excess Yku binding and promotes the recruitment of Exo1 and Dna2/Sgs1 to process the break ends to generate long ssDNA. Then Rad51 replaces RPA complex to mediate homologous template searching and repair of DSB.