Figure 4.

In the absence of mS38, COX1 mRNA is loaded onto the mitoribosomes but is poorly detected in polysomes. (A) Scheme depicting the experimental protocol for mito-polysome profiling. (B) Sucrose gradient sedimentation analyses of mitoribosomal subunits, monosomes, and polysomes in extracts obtained from mitochondria isolated from the indicated strains. One representative image of three independent experiments is shown. The graphs in the lower panels represent the relative amount of the indicated rRNAs and mRNAs in the gradient fractions, estimated by RT-qPCR. The bars represent the average ± SD of three independent repetitions.