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. 2019 Jun 12;10:642. doi: 10.3389/fphar.2019.00642

Figure 7.

Figure 7

Transwell permeability assay. HUVECs were seeded onto Transwell inserts and infected with PRVABC59 or IBH30656 ZIKV (MOI 0.1) or mock. FITC-dextran was added into the upper compartment of the Transwell system. Aliquots of culture medium from the lower chamber were collected at selected time points to determine the presence of dextran molecule. Data are presented as a percent change in permeability as compared to mock-infected control. MMPs were inhibited using GM6001 (10 µM). Also, GM6001 negative control (10 µM) was used. (A) FITC-dextran permeability of HUVECs infected with PRVABC59 or IBH30656 ZIKV; (B) FITC-dextran permeability of HUVECs infected with PRVABC59 or IBH30656 ZIKV and treated with GM6001; (C) FITC-dextran permeability of HUVECs infected with PRVABC59 or IBH30656 ZIKV and treated with GM6001, negative control. ***p < 0.0001 by paired t test. Data shown are the mean ± Sterr of three independent experiments.