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NEAT1 affects NP ECM metabolism. (a) Expression of NEAT1 in degenerative NP cells transfected with pcDNA3.1-NEAT1 (NEAT1) or the control plasmid was determined with qRT-PCR. (b–e) mRNA expression levels of ADAMTS-4, aggrecan, MMP-13, and type II collagen were determined after NEAT1 overexpression. (f) Effects of NEAT1 overexpression on ADAMTS-4, aggrecan, MMP-13, and type II collagen protein expression. Data represent the mean ± SD. *P < 0.05 vs. control group, n = 3.
