Table 1.

Two-way ANOVA P-values.

	Day	ECMRigidity	TGF-β1	Interaction
Cell density	1	0.5316	0.8926	0.7083
	3	0.3082	0.1459	0.8041
	5	0.6812	0.1569	0.9875
Actin coverage	1	0.1268	0.8791	0.7008
	3	0.3607	<0.0001*	0.7373
	5	0.7807	<0.0001*	0.9068
α-SMA/actin coverage	1	0.0655	<0.0001*	0.5635
	3	0.0474*	<0.0001*	0.0283*
	5	0.3342	<0.0001*	0.1021
ACTA2 relative expression	1	0.3255	<0.0001*	0.4763
	3	0.6160	<0.0001*	0.7983
POSTN relative expression	1	0.8103	<0.0001*	0.2997
	3	0.6836	0.1872	0.4287
FAP relative expression	1	0.8928	0.0032*	0.3781
	3	0.4506	0.0016*	0.0670
FSP1 relative expression	1	0.5935	<0.0001*	0.1548
	3	0.5417	0.0172*	0.5088
GJA1 relative expression	1	0.8300	0.8398	0.2188
	3	0.0953	0.9058	0.0609

Note: Data for all conditions were normally distributed, as determined by the Kolmogorov–Smirnov test (n = 4, *P < 0.05).

ECM: extracellular matrix.