Figure 3.

Result validation using RT-PCR. RNA was isolated from CGN cultures infected with Ad-GFP or Ad-HDAC3. Expression of 15 of the 21 differentially expressed proteins was examined by RT-PCR analyses. mRNA level changes for six genes, including Ppp2r2d, Smarca2, Gpd1, Nptx1, Plxnb1, and Sec62, displayed consistent patterns with proteomic findings. The nine other proteins along with HDAC9 showed no significant changes at mRNA levels. Hip1r showed a dramatic downregulation by HDAC3. HDAC3, c-Jun, and Actin were run as controls for sample quality. For each of the genes, left panel: GFP control sample; right panel: HDAC3-overexpressing sample.