Figure 5.

Protein/gene regulation by HDAC3 in HEK293T cells. HEK293T cells were transfected with HDAC3-FLAG or GFP plasmid and then 30 h later harvested for mRNA or protein lysates. (a) Protein lysates were analyzed by Western blotting using antibodies to proteins shown in the figure. Western blot analysis showed that protein levels of c-JUN, PSME2, PLXNB1, and HDAC9 significantly increased in HDAC3-overexpressing HEK293T cells compared to GFP control. No significant changes were observed from other proteins. (b) RNA was subjected to RT-PCR analysis using primers against the mRNA shown in the figure. Expression of GPD1 mRNA was dramatically upregulated by HDAC3 in HEK293T cells, whereas levels of HIP1R and PTPRN were downregulated. No significant changes were found in other genes. For each of the genes, left panel: GFP control sample; right panel: HDAC3-overexpressing sample.