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Optimal biochemical reaction conditions for the RNA helix unwinding activity of EBOV VP35. (A) Schematic illustration of the standard RNA helix substrate (R*/R). Asterisks indicate the HEX-labeled strands. (B–D) MBP–VP35 (20 pmol) was reacted with 0.1 pmol standard RNA helix substrate in the presence of each indicated divalent metal ion (2 mM for each) (B), increasing concentrations of Mg2+ (C), or indicated pH values (D).
