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EBOV VP35 unwinds RNA-protruded nucleic acid helices. (A–D) MBP–VP35 (20 pmol) was reacted with 0.1 pmol of standard RNA helix (R*/R) (A), DNA helix (D*/D) (B), or DNA/RNA hybrid with longer RNA strand (D/R*) (C) or longer DNA strand (R*/D) (D). For (A–D), schematic illustrations of the helix substrates are illustrated in the upper panels. Asterisks indicate the HEX-labeled strand.
