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. 2019 Mar 14;47(11):e61. doi: 10.1093/nar/gkz171

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© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 8. — Assay for alkyladenine DNA glycosylase (AAG) activity. Incubation with recombinant human AAG led to enzyme concentration- (A) and time-dependent (B) decreases in the amount of intact oligonucleotide substrate containing an I:T base pair following alkaline denaturation. For the time course analysis squares, circles and triangles indicate data generated with 0.1, 0.2 or 0.4 units/well, respectively. Incubation with HepG2 nuclear extract (C) or peripheral blood mononuclear cell pooled extract (D) also caused concentration-dependent decreases in the amount of intact substrate retained in the wells. The apparent AAG activity was directly proportional to the sample concentration over the range 0–10 μg/well for HepG2 (E) and peripheral blood mononuclear cell (F) nuclear extracts (r² = 0.94 in both cases). Data shown represent the mean ± SD of triplicate technical replicates except for time course data for which duplicates were analyzed.