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. 2019 Jun 15;21(3):274–280. doi: 10.22074/cellj.2019.6239

Fig 3.

Fig 3

The effect of MSCs administration on the immunophentype of uDCs. The uterine cells were isolated from uterine of MSC-treated, un-treated group (controlgroup) and normal pregnant (normal-pregnancy) mice at the gd 13.5, stained with monoclonal antibodies against CD45, CD11c and one of the monoclonal antibodies (anti-MHC-II, anti-CD86, anti-CD11b and anti-CD40) then analyzed by flow cytometry. A. The CD11c positive uDCs were selected from the CD45+ cells of whole uterine cell population. Then the expression of CD11b, CD86, CD40 and MHC-II on uDCs was evaluated .The red histograms show the isotype controls and B. The graphs indicate that MSCs administration significantly decreased the expression of MHC-II and co-stimulatory molecules (CD86, CD40) on uDCs while CD11b+ DCs were not changed following MSC therapy. The differences between the groups were evaluated using a standard parametric test (one-way ANOVA test). The results show the mean ± SD of five independent experiments. **, ***, and ****; p<0.01, p<0.001, and p<0.0001 respectively, MSCs; Mesenchymal stem cells, uDC; Uterine dendritic cells, and NS; Not significant.