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. 2019 Jun 18;19:134. doi: 10.1186/s12906-019-2561-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effects of CG on extrinsic apoptosis pathway-related factors in A549 and NCI-H1299 cells. mRNA expression of DR5, FADD, Fas, and FasL in A549 (a) and NCI-H1299 (b) cells, as determined by qPCR analysis. The graph was compiled from at least three replicate analyses. Protein expression of the extrinsic pathway factors, pro-caspase-8 and its cleaved form, in A549 (c) and NCI-H1299 (d) cells, as determined by western blotting. The cells were treated with various concentrations of CG for 48 h and compared with untreated cells. The data are presented as the mean ± SEM (n = 3). The data were analyzed using one-way ANOVA with Tukey’s HSD test. *, p < 0.05 and **, p < 0.005. Pro cas-8, pro-caspase-8; Cleav-cas-8, cleaved caspase-8