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. 2019 Jun 18;19:134. doi: 10.1186/s12906-019-2561-1

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — ROS generation induced by CG in A549 and NCI-H1299 cells. CG treatment resulted in ROS generation in A549 (a) and NCI-H1299 (b) cells. Cells were treated with CG for 48 h and examined using DCF-DA staining and a fluorescence microplate reader. The mRNA expression of SOD2 and GAPDH was determined by PCR analysis in A549 (c) and NCI-H1299 (d) cells treated with CG extract for 48 h. Western blots of SOD2 and GAPDH protein expression in CG-treated A549 (e) and NCI-H1299 (f) cells. The data are presented as the mean ± SEM (n = 3). The data were analyzed using one-way ANOVA with Tukey’s HSD test. *, p < 0.05 and **, p < 0.005