Table 1.
Effect of cryopreservation on the motility parameters of human sperm frozen in cryostraw and cryovial
| Group | Control (C) | Cryostraw (CS) | Cryovial (CV) | p value | ||
|---|---|---|---|---|---|---|
| C:CS | C:CV | CS:CV | ||||
| MOT (%) | 81.83 ± 5.71a | 19.10 ± 4.67b | 27.55 ± 9.60c | 9.37E−18 | 3.55E−16 | 0.01 |
| FAR (%) | 63.36 ± 7.65a | 9.72 ± 3.43b | 15.48 ± 6.17c | 1.06E−17 | 1.85E−16 | 0.04 |
| VCL (μm/s) | 39.26 ± 6.26a | 27.69 ± 3.98b | 27.78 ± 2.73b | 5.11E−06 | 5.75E−06 | 0.97 |
| VSL (μm/s) | 14.49 ± 2.93a | 9.31 ± 1.75b | 9.64 ± 1.87b | 2.00E−05 | 4.83E−05 | 0.75 |
| VAP (μm/s) | 23.95 ± 3.43a | 16.19 ± 2.18b | 16.29 ± 2.13b | 5.07E−07 | 6.31E−07 | 0.93 |
| LIN (%) | 37.13 ± 5.45 | 33.62 ± 4.22 | 34.49 ± 4.13 | 0.10 | 0.21 | 0.68 |
| STR (%) | 60.33 ± 5.62 | 57.27 ± 6.63 | 58.77 ± 4.87 | 0.24 | 0.55 | 0.56 |
| VIB (%) | 61.31 ± 3.99 | 58.78 ± 4.67 | 58.59 ± 3.83 | 0.19 | 0.16 | 0.92 |
| ALH (μm) | 2.09 ± 0.31a | 1.89 ± 0.28ab | 1.82 ± 0.14b | 0.09 | 0.03 | 0.54 |
Different superscripts within a row indicate significant differences (p < 0.05)
MOT motile sperm; FAR forward activity rate; VCL curvilinear velocity; VSL straight line velocity; VAP average path velocity; LIN linearity; STR straightness index; VIB vibration index; ALH amplitude of lateral head displacement