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. 2019 Jun 18;10:178. doi: 10.1186/s13287-019-1292-z

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Anti-apoptotic effects of calystegines on EMS_ASCs cells. a Flow cytometry-based Annexin V/7-AAD representative plots. b Bar chart representation of total living and total apoptotic cell distribution expressed in percentage. c RT-qPCR analysis of relative expression of the main related apoptotic genes. Ctrl_HE healthy control cells, Ctrl_EMS EMS control cells, EMS_Cal 250 EMS cells treated with 250 μg/ml of calystegines, EMS_Cal 500 EMS cells treated with 500 μg/ml of calystegines. Values are mean ± SD (n = 3). Asterisk (*) refers to the comparison of EMS treated and untreated groups to untreated healthy cells, Hashtag (#) refers to the comparison of EMS treated groups to EMS untreated cells. *^/#p < 0.05, **^/##p < 0.01, ***^/###p < 0.001