Fig. 7.

RT-PCR analysis of insulin resistance markers after calystegine treatment on EMS_ASCs cells. a Gene expression levels of key factors involved in insulin signaling pathway. b Gene expression levels of lipid metabolism markers. Ctrl_HE healthy control cells. Ctrl_EMS EMS control cells, EMS_Cal 250 EMS cells treated with 250 μg/ml of calystegines, EMS_Cal 500 EMS cells treated with 500 μg/ml of calystegines. Results are expressed as mean of three independent experiments ± SD. Asterisk (*) refers to the comparison of EMS treated and untreated groups to untreated healthy cells. Hashtag (#) refers to the comparison of EMS treated groups to EMS untreated cells. *^/#p < 0.05, **^/##p < 0.01, ***^/###p < 0.001