Table 1.
Sequences of primers used in qPCR
| Gene | Primer | Sequence 5′–3′ | Amplicon length (bp) | Accession no. |
|---|---|---|---|---|
| GLUT4 | F: | TGGGCTCTCTCCGTGGCCATCTT | 658 | NM_001081866.2 |
| R: | GCTGCTGGCTGAGCTGCAGCA | |||
| Akt | F: | AAGGAGATCATGCAGCACCG | 180 | XM_014854427.1 |
| R: | CTCCATCGTGTCGTCTTGGT | |||
| PI3K | F: | GACTTGCACTTGGGTGACATA | 152 | XM_014855332.1 |
| R: | TAAGTTCCCGGAAAGTCCCC | |||
| IR | F: | CCGTTTGAGTCTGAGGGGTC | 254 | XM_014862015.1 |
| R: | ACCGTCACATTCCCGACATC | |||
| IRS | F: | CTGCTGGGGGTTTGGAGAAT | 254 | XM_014862015.1 |
| R: | TAAATCCTCACTGGAGCGGC | |||
| SREBP-1c | F: | TCAGCGAGGCGGCTTTGGAGCAG | 80 | XM_008542859.1 |
| R: | CATGTCTTCGATGTCGGTCAG | |||
| PPARγ | F: | TCCCTGTTTGTGTACAGCCTT | 191 | XM_014846252.1 |
| R: | CTCCATGGCTGATTTCCCCT | |||
| ADIPOQ | F: | GGAGATCCAGGTCTTGTTGG | 162 | XM_014843352.1 |
| R: | TCGGGTCTCCAATCCTACAC | |||
| Lep | F: | CACACGCAGTCAGTCTCCTC | 176 | XM_014854289.1 |
| R: | CGGAGGTTCTCCAGGTCAT | |||
| p53 | F: | TACTCCCCTGCCCTCAACAA | 252 | U37120.1 |
| R: | AGGAATCAGGGCCTTGAGGA | |||
| Cas-9 | F: | CACCTTCCCAGGCTTTGTCT | 224 | XM_014836232.1 |
| R: | GGCTCTGGCCTCAGTAAGTT | |||
| Bax | F: | TTCCGACGGCAACTTCAACT | 204 | XM_005596728.1 |
| R: | GGTGACCCAAAGTCGGAGAG | |||
| Bcl-2 | F: | TTCTTTGAGTTCGGTGGGGT | 164 | XM_014843802.1 |
| R: | GGGCCGTACAGTTCCACAA | |||
| p21 | F: | GAAGAGAAACCCCCAGCTCC | 241 | XM_003365840.2 |
| R: | TGACTGCATCAAACCCCACA | |||
| PERK | F: | GTGACTGCAATGGACCAGGA | 283 | XM_014852775.1 |
| R: | TCACGTGCTCACGAGGATATT | |||
| CHOP | F: | AGCCAAAATCAGAGCCGGAA | 272 | XM_014844003.1 |
| R: | GGGGTCAAGAGTGGTGAAGG | |||
| BiP | F: | CTGTAGCGTATGGTGCTGCT | 122 | XM_023628864.1 |
| R: | CATGACACCTCCCACGGTTT | |||
| eIF2-α | F: | AGTCTTCAGGCATTGGCTCC | 489 | XM_001488848.5 |
| R: | CCGAGTGGGACATGTATCGG | |||
| LC3 | F: | TTCTGAGACACAGTCGGAGC | 128 | XM_001493613.6 |
| R: | CTTTGTTCGAAGGTGTGGCG | |||
| Beclin | F: | GATGCGTTATGCCCAGATGC | 233 | XM_014833759.1 |
| R: | AACGGCAGCTCCTCTGAAAT | |||
| Lamp2 | F: | GCACCCCTGGGAAGTTCTTA | 147 | XM_014831347.1 |
| R: | ATCCAGCGAACACTCTTGGG | |||
| GAPDH | F: | GATGCCCCAATGTTTGTGA | 250 | NM_001163856.1 |
| R: | AAGCAGGGATGATGTTCTGG |