Fig. 3.
Effects of different concentrations of recombinant SAA1 protein on NOX4/ROS pathway in LPS-induced VSMCs (n = 3). Notes: The levels of superoxide anion (O2−) production (a), NADPH oxidase activity (b), and NOX-4 protein expression (c-d) in VSMCs after pre-incubation with different concentrations of SAA (5, 10, 15, 20 μg/ml) for 24 h followed by the addition with 1 μg/ml LPS for 24 h; Some VSMCs were treated with SAA (20 μg/ml) alone for 24 h. The same letters represented no significant differences, P > 0.05; and different letters indicated significant differences, P < 0.05