Table 2.
The sequence of primers, RNA oligo in this study
| Sequence (5′ to 3′) | |
|---|---|
| Primers | |
| hsa-miR-6721-5p |
Forward: GATTGGGCAGGGGCTTATTG Reverse: CAGTGCAGGGTCCGAGGTAT |
| hsa-miR-20a-3p |
Forward: CGGAGACTGCATTATGAGCAC Reverse: CAGTGCAGGGTCCGAGGTAT |
| hsa-miR-330-5p |
Forward: GCCTCTCTGGGCCTGTGTC Reverse: CAGTGCAGGGTCCGAGGTAT |
| hsa-miR-326 |
Forward: CATCGCCTCTGAGCCCTTC Reverse: CAGTGCAGGGTCCGAGGTAT |
| hsa-miR-4527 | Forward: AGGTGGTCTGCAAAGAGATGA |
| Reverse: CAGTGCAGGGTCCGAGGTAT | |
| hsa-miR-6503-5p | Forward: GCGAGGTCTGCATTCAAATCC |
| Reverse: CAGTGCAGGGTCCGAGGTAT | |
| hsa-miR-432-5p | Forward: GGCTCTTGGAGTAGGTCATTGG |
| Reverse: CAGTGCAGGGTCCGAGGTAT | |
| hsa-miR-7150 | Forward: GGTAGACTGGCAGGGGGAG |
| Reverse: CAGTGCAGGGTCCGAGGTAT | |
| hsa-miR-4260 | Forward: GCAGACTTGGGGCATGGA |
| Reverse: CAGTGCAGGGTCCGAGGTAT | |
| U6B | Forward: CGCTTCGGCAGCACATATAC |
| Reverse: AAAATATGGAACGCTTCACGA | |
| RNA oligo | |
| miR-330-5p mimics |
AAGCUGCCAGUUGAAGAACUGU ACAGUUCUUCAACUGGCAGCUU |
| miR-330-5p mimics NC |
GCAACCUGUUAGACUAUGGAGA UCUCCAUAGUCUAACAGGUUGC |
| Talin-1 |
GUCCAUCAUUCAUGCGAAATT UUUCGCAUGAAUGAUGGACTT |
| Negative control |
UUCUCCGAACGUGUCACGUTT ACGUGACACGUUCGGAGAATT |
| GAPDH Positive control |
UGACCUCAACUACAUGGUUTT AACCAUGUAGUUGAGGUCATT |