Fig. 1.

The osteoblastic differentiation and miR-34a expression of irradiated BMSCs. a ALP staining after osteogenic induction for 7 days. b Alizarin red staining after osteogenic induction for 21 days, scale bar = 1 cm. c Gene expression of Runx2, Alp, Col-1, and Ocn after 14 days of osteogenic induction. d Western blot analysis of RUNX2, ALP, COL-1, OCN, and GAPDH after 14 days of osteogenic induction. e The quantitative analysis of the Western blot results relative to GAPDH (fold to control). f Expression of miR-34a during osteoblastic differentiation. g Gene expression of Runx2, Alp, Col-1, and Ocn after 14 days of osteogenic induction in the BMSCs isolated from non-irradiated (N-BMSC) and irradiated tibias (IR-BMSC). h Expression of miR-34a of N-BMSC and IR-BMSC during osteogenic differentiation. Data are shown as mean ± SD, n = 3; *p < 0.05, **p < 0.01, ***p < 0.001